Very—low-density lipoprotein apolipoprotein B100 kinetics in adult hypopituitarism

Abstract

Hypopituitarism is associated with hyperlipidemia, the mechanisms of which are not fully known. One possible mechanism is an increased hepatic secretion of very—low-density lipoprotein (VLDL) apolipoprotein B100 (apo B100). To investigate this, 13 hypopituitary patients (seven women and six men; age, 46 ± 3 years [mean ± SEM]; body mass index [BMI], 29 ± 2 kg/m 2) and 13 matched controls (seven women and six men; age, 43 ± 3 years; BMI, 28 ± 2 kg/m 2) were investigated in a stable-isotope study. [1- 13C]leucine (1 mg/kg body weight) was administered, followed by a continuous 6-hour infusion of [1- 13C]leucine (at a rate of 1 mg/kg/h). Patients had a similar fractional secretion rate (FSR) of VLDL apo B100 versus controls (0.37 ± 0.05 v 0.38 ± 0.06 pools/h, respectively), but they had a significantly larger pool size (3.4 ± 0.3 v 1.9 ± 0.3 mg/kg) and higher absolute secretion rate ([ASR] 27.8 ± 2.9 v 16.0 ± 2.5 mg/kg/d). The increase in hepatic VLDL production may explain the lipid abnormalities found in hypopituitarism. Fasting circulating nonesterified fatty acids (NEFAs) were decreased in the patients (284 ± 26 v 664 ± 92 μmol/L, P < .001) despite the increase in VLDL secretion. An inverse relationship was observed between the NEFA level and VLDL apo B100 FSR in the patients ( r s = − .85, P < .005).