Abstract
A capillary zone electrophoresis (CZE) method was investigated for the determination of creatinine and creatine in human urine by using a fused-silica capillary (75μm ID × 30cm total length, 10cm effective length). The separation was performed using an hydrodynamic injection time of 3s (0.5psi), a voltage of −15kV and a 60mM phosphoric buffer solution at pH 2.2 as electrolyte separation. Under these conditions, the analysis takes less than 2.7min. A linear response over the 3.0–120mgL−1 concentration range was investigated for compounds. A dilution of the sample (water:urine) was the only step necessary before the electrophoresis analysis. Detection limits of 0.7 and 1.3mgL−1 for creatinine and creatine (S/N=3) were obtained. The developed method is easy, rapid and sensitive and has been applied to determine creatinine and creatine in different human urine samples.
Published Version
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