Vertical zonation of soil fungal community structure in a Korean pine forest on Changbai Mountain, China.

Abstract

Changbai Mountain, with intact montane vertical vegetation belts, is located at a sensitive area of global climate change and a central distribution area of Korean pine forest. Broad-leaved Korean pine mixed forest (Pinus koraiensis as an edificator) is the most representative zonal climax vegetation in the humid region of northeastern China; their vertical zonation is the most intact and representative on Changbai Mountain. In this study, we analyzed the composition and diversity of soil fungal communities in the Korean pine forest on Changbai Mountain at elevations ranging from 699 to 1177m using Illumina High-throughput sequencing. We obtained a total 186,663 optimized sequences, with an average length of 268.81bp. We found soil fungal diversity index was decreased with increasing elevation from 699 to 937m and began to rise after reaching 1044m; the richness and evenness indices were decreased with an increase in elevation. Soil fungal compositions at the phylum, class and genus levels varied significantly at different elevations, but with the same dominant fungi. Beta-diversity analysis indicated that the similarity of fungal communities decreased with an increased vertical distance between the sample plots, showing a distance-decay relationship. Variation partition analysis showed that geographic distance (mainly elevation gradient) only explained 20.53% of the total variation of fungal community structure, while soil physicochemical factors explained 69.78%.