Abstract
La Crosse (LAC) virus remained infective to warm-blooded hosts after 8 successive transovarial passages in the mosquito Aedes triseriatus . Virtually all transovarially infected females (98%) transmitted LAC virus to their offspring, and the mean percentage of infected offspring from an infected female was 71%, a rate that remained essentially constant from one generation to the next. These results indicate that the virus can persist 4 years or longer in the absence of horizontal amplification in vertebrate hosts and the data provide a basis for estimating, during anyone season, the amount of amplification necessary to maintain the virus at existing endemic levels.
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