Vertical profiles of current velocity and dissolved oxygen saturation in biofilms on artificial and natural substrates

Abstract

We determined vertical changes in current velocity and dissolved oxygen concentration in biofilms on artificial and natural substrates using microelectrodes. We used biofilms developed on glass slides dipped in an artificial stream for 3 months, artificial clay tiles dipped in an outdoor artificial stream for 3 months, and natural pebbles. In the biofilm on a glass slide, current velocity significantly decreased from the surface of the biofilm and became 0 cm s−1 at the surface of the glass slide. Vertical profile of current velocity versus depth indicated a presence of a viscous sublayer of 0.2-mm thickness above the surface of glass slide. Dissolved oxygen (DO) concentration increased within the biofilm and attained the maximum (123%) at the surface of the glass slide, indicating active photosynthesis by sessile diatoms at the layer corresponding to the observed viscous sublayer. In the biofilm on an artificial tile, DO increased to 163% saturation at 24.849-mm depth, followed by rapid decrease (6%) at the surface of the tile. A similar result of remarkable decrease in DO saturation was also found in the biofilm on a natural pebble. These results suggest that smoothness of the substrate surface is related to the vertical profile of DO saturation. The thickness of the viscous sublayer and oxygen-depleted area (up to several hundred micrometers, μm) was sufficient for the presence of bacteria, protists, and other metazoan animals, suggesting high activity and diversity of those heterotrophs in the bottom part of biofilms.