Vertical distribution of microbial communities in soils contaminated by chromium and perfluoroalkyl substances

Abstract

Both Bacteria and Archaea are important players in soil biogeochemical cycles. Both chromium (Cr) and perfluoroalkyl substances (PFASs) are widely present in soil environment. However, the depth-related distribution of microbial community in soils contaminated by Cr or/and PFASs remains unknown. Hence, the present study applied quantitative PCR assay and Illumina MiSeq sequencing to investigate the vertical variations of archaeal and bacterial communities in soils (0.5–12.5m depth) contaminated by chrome plating waste and the potential effects of Cr and PFASs.Both bacterial and archaeal communities displayed the remarkable depth-related changes of abundance (2.16×107–5.05×109 and 4.95×105–2.56×108 16S rRNA gene copies per gram dry soil respectively for Bacteria and Archaea), diversity (bacterial and archaeal Shannon diversity indices of 5.06–6.34 and 2.91–4.61, respectively) and structure. However, at each soil depth, bacterial community had higher abundance, richness and diversity than archaeal community. Soil bacterial communities were mainly composed of Proteobacteria, Chloroflexi, Actinobacteria and Firmicutes, and archaeal communities were dominated by Thaumarchaeota and unclassified Archaea. Moreover, microbial abundance and richness increased with increasing perfluorohexane sulfonate (PFHxS) content. Microbial abundance was correlated to total Cr, and archaeal richness was correlated to total Cr and Cr(IV). In addition, total Cr might be a key determinant of soil microbial community structure.