Vertical and temporal variations in activity, abundance, and composition of nitrite-driven anaerobic methanotrophs in a paddy field

Abstract

Paddy soil is an important source of methane (CH4) emissions, and the nitrite-driven anaerobic oxidation of methane (AOM) mediated by Candidatus Methylomirabilis bacteria is a crucial process for CH4 mitigation. However, the vertical and temporal fluctuations in nitrite-driven AOM activity, as well as the abundance and composition of Methylomirabilis bacteria in paddy soil, remain poorly understood. We investigated the potential rate of nitrite-driven AOM and the composition of Methylomirabilis bacteria at varying depths (0–40 cm, at 10 cm intervals) within a paddy soil during different growth stages of rice, including tillering, jointing, flowering, and milky. The potential rate of nitrite-driven AOM ranged from 0.24 to 20.6 nmol CO2 g−1 (dry soil) d−1, and the abundance of Methylomirabilis bacterial 16S rRNA genes varied from 5.8 × 106 to 4.4 × 107 copies g−1 dry soil. The 10–20 cm soil depth showed higher potential activity and bacterial abundance compared to other depths, and the tillering and jointing stages exhibited greater activity and abundance. The composition of Methylomirabilis bacteria showed significant variation across different soil depths but remained stable throughout the growth stages of rice. Soil organic carbon content was positively correlated with potential nitrite-driven AOM rate, and nitrite content was negatively correlated with the abundance of Methylomirabilis bacteria. Soil organic carbon and ammonium content had a significant impact on the composition of Methylomirabilis bacteria. The results underscore the substantial influence of soil depth and the growth stage of rice on nitrite-driven AOM in paddy ecosystems.