Vertebral Body Adherent Allogeneic Mesenchymal Stromal Cells Increase in Vivo Perfusion and Improve Muscle Function in Diabetic Mouse Models of Critical Limb-threatening Ischemia

Abstract

Critical limb-threatening ischemia (CLTI) is a health care priority; since 2010, there has been a 50% increase of non-traumatic major amputations. We have previously shown that autologous bone marrow cell therapy is not effective in preventing amputations on patients with poor options for revascularization. Allogeneic mesenchymal stromal cells (MSCs) from young healthy donors may have greater therapeutic potential, particularly in diabetic populations. Here, we describe the effects of intramuscular injection of vertebral body adherent (vBA)-MSCs in restoring limb perfusion, muscle regeneration and limb function in two diabetic CLTI mouse models.