Abstract
In order to find a high-speed countercurrent chromatography solvent system that can be used as a general prefractionation step for anthraquinone, the crude extracts of five traditional Chinese medicines, Radix rubiae, Rhizoma polygoni cuspidati, Semen cassiae, Radix et rhizoma rhei, and Aloe were separated with a two-phase system composed of CHCl3:CH3OH:H2O = 4:x:2 in which x was between 3 and 4 for different samples. The fractionated components were identified on TLC. This solvent system gave a good separation in most of the anthraquinones.
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