Abstract
Tracer methods using both carbon-13 and -14 have been utilized for determination of ovine fetal amino acid disposal and the results compared in seven animals. We infused [1- 13C]leucine simultaneously with [1- 14C]leucine into the fetal circulation of pregnant sheep chronically catheterized during late gestation. Radio-active and stable isotope enrichments of leucine (Leu) and stable isotope enrichments of ketoisocaproic acid (KIC) in the umbilical artery and vein and the maternal artery and uterine vein were measured. Stable isotope enrichments and concentrations of both Leu and KIC were determined from a single 0.2-ml sample by the use of internal standards and electron ionization GC MS analysis after a simple isolation and derivatization procedure. The KIC Leu enrichment ratio was measured for the first time in fetal arterial plasma and was 0.66 ± 0.05 (SE). Fetal leucine disposal rate was 9.0 ± 0.5 (SE) μm/min/kg. Disposal rates determined by stable isotopes were not different from those determined by radioactive isotopes. The GC MS stable isotope method provided higher precision in both leucine concentration and enrichment measurements and has been shown to be a general method for the determination of concentration and isotopic enrichment of other amino acids and their corresponding keto acids. Furthermore, this method is ideally suited to clinical studies where large numbers of samples of rather small volume can easily be studied with a short turnaround time.
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