Versatile Multiplexed Super-Resolution Imaging of Nanostructures by Quencher-Exchange-Paint

Tobias Lutz,Alexander H Clowsley,Ruisheng Lin,Stefano Pagliara,Lorenzo Di Michele,Christian Soeller

doi:10.1016/j.bpj.2017.11.1946

Tobias Lutz, Alexander H Clowsley + Show 4 more

Open Access

https://doi.org/10.1016/j.bpj.2017.11.1946

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Abstract

The work was supported by funding from the Human Frontier Science Program (No. 0027/2013) and the Engineering and Physical Sciences Research Council of the UK (No. EP/N008235/1).

Highlights

1729-Pos Board B638 Versatile Multiplexed Super-Resolution Imaging of Nanostructures by Quencher-Exchange-Paint Tobias Lutz1, Alexander H
The solution exchange of imager strands is the basis of Exchange-PAINT which enables multiplexed imaging that avoids chromatic aberrations
Quencher-Exchange-PAINT uses oligonucleotides which inhibit the imager from binding to the targets, ‘quencher’ strands, to rapidly reduce unwanted single-stranded imager concentrations to negligible levels and decoupled from absolute imager concentration

Summary

Introduction

1729-Pos Board B638 Versatile Multiplexed Super-Resolution Imaging of Nanostructures by Quencher-Exchange-Paint Tobias Lutz1, Alexander H. This technique reduced the spatial resolution to &lt 10 nm, in certain cases this high resolution is not required and the size of an image in AFM-IR is limited by the piezo stage to sub 100 mm.

Results

Conclusion