Versatile chiral chromatography with mixed stationary phases of water-impregnated silica gel and reversed-phase packing

Abstract

A novel chiral chromatographic scheme is proposed, which requires no organic syntheses in stationary phase preparation. A normal phase chiral chromatography stationary phase is prepared by simple passage of an aqueous solution of an appropriate chiral selector through e.g. a silica gel column. For this scheme, a mixed-bed of bare silica gel and octadecylsilanized silica gel (ODS) provides much better separation performance than a silica gel column. Isolation of silica gel particles in the column is important for successful chiral separation based on the present scheme.