Verification of the utility of molecular markers linked to the multiple-allele male-sterile gene Ms in the breeding of male-sterile lines of Chinese cabbage (Brassica rapa)

Abstract

To verify the molecular markers linked to the genic multiple-allele male-sterile gene Ms , an F 1 plant, which was generated by crossing the inbred line a20 and the male-sterile plant of the genic multipleallele male-sterile AB line, was backcrossed with an a20 plant to develop BC 4 and BC 5 populations. Sequence-characterized amplified region (SCAR) marker syau_scr01 and simple sequence repeat (SSR) marker syau_m13 , which were linked to Ms, exhibited polymorphism between the 2 parents. The accuracies of these 2 markers in determining the plant genotype was 85 and 91.7%, respectively. The accuracy reached 100% when the 2 markers were used in combination. These results indicate that these 2 markers can be applied in the marker-assisted selection of the genic multiple-allele male-sterile line of Chinese cabbage. Keywords: Chinese cabbage, genic multiple-allele male sterility, marker-assisted selection, simple sequence repeat, sequence-characterized amplified region African Journal of Biotechnology Vol. 9(35), pp. 5623-5628, 30 August, 2010