Verification of the analgesic properties of natural compounds of cannabinoid origin in Danio rerio model of nociception

Abstract

The use of Cannabis sativa in human history dates back thousands of years, with various historical and cultural applications. However, at the beginning of the 20th century, many countries enacted regulations to criminalize and restrict the use of cannabis, leading to a significant reduction in research on its medical applications. A novel approach to pain studies involves Danio rerio-based nociception models. These models use different methods to induce pain, with fish larvae often subjected to incubation in acetic acid solution, resulting in epidermal tissue damage. Nociceptive responses are then observed by tracking fish movement.Our research aimed to develop a simple and accessible Danio rerio (zebrafish) model of nociception to study the potential analgesic properties of CBD (cannabidiol) and CBG (cannabigerol) in comparison to the commonly known painkiller ibuprofen. This research seeks to contribute to our understanding of the potential therapeutic applications of cannabinoids in pain management.