Abstract
AbstractThe plasmid ColE1 and its derivatives are the most commonly used vectors in gene cloning experiments. A very often used method to obtain a sufficient amount of plasmid DNA is to treat exponential growing cells of Escherichia coli containing plasmids with chloramphenicol [2]. An amplification of these plasmids is also possible in Escherichia coli relA mutants if one of the necessary amino acid limits the growth [8]. On the basis of this system a fed‐batch fermentation process was developed with a ten times higher yield of plasmid DNA than the method based on chloramphenicol.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.