Abstract
AbstractThe plasmid ColE1 and its derivatives are the most commonly used vectors in gene cloning experiments. A very often used method to obtain a sufficient amount of plasmid DNA is to treat exponential growing cells of Escherichia coli containing plasmids with chloramphenicol [2]. An amplification of these plasmids is also possible in Escherichia coli relA mutants if one of the necessary amino acid limits the growth [8]. On the basis of this system a fed‐batch fermentation process was developed with a ten times higher yield of plasmid DNA than the method based on chloramphenicol.
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