Verapamil and aspirin modulate platelet-mediated vasomotion in arterial segments with intact or disrupted endothelium

Abstract

Objectives. This study was designed to examine the effects of verapamil and aspirin, which decrease thromboxane A2and serotonin release, on the modulation of vascular tone by platelets. Background. Aggregating platelets cause constriction of deendothelialized arterial segments through thromboxane A2and serotonin release. These cells cause relaxation of arterial segments with intact endothelium through release of the endotheliumderived relaxing factor.Methods. Healthy subjects were given either no drug, verapamil or aspirin for ≥5 days before their platelets were obtained. The effects of platelets obtained from subjects before and after treatment with aspirin or verapamil on the tone of rat aortic rings were determined.Results. As expected, control platelets (before verapamil or aspirin treatment) induced concentration-dependent relaxation of rat aortic rings with intact endothelium and a concentrationdependent contraction of de-endothelialized rings. Verapamil treatment enhanced (p < 0.02) the platelet-mediated relaxation in rings with intact endothelium and abolished platelet-mediated constriction (p < 0.01) in the de-endothelializcd rings. Aspirin treatment also abolished (p < 0.05) platelet-mediated constriction of the de-endothelialized rings. The de-endothelialized rings contracted normally in response to the synthetic thromboxane A2analogue U46, 619, as well as to serotonin, indicating that the vascular smooth muscle response to thromboxane A2and serotonin was intact.Conclusions. This study provides evidence for the modulation of platelet-mediated vasoconstriction of de-endothelialized arterial segments by prior treatment of subjects with verapamil or aspirin. In clinical syndromes characterized by endothelial dysfunction or disruption, treatment with verapamil or aspirin may modify platelet-vessel wall interactions.