Venom of the parasitoid wasp Pteromalus puparum contains an odorant binding protein.

Abstract

Odorant binding proteins (OBPs) are crucial for insects to detect food, mates, predators, or other purposes. They are mostly located on antennae and other olfactory sensilla. In this study, we identified an OBP from the venom of Pteromalus puparum, designated as PpOBP. The cDNA of PpOBP is 517 bp in length, encoding 132 amino acids. Phylogenetic analysis revealed that PpOBP was clustered with OBP68 and OBP67 of Nasonia vitripennis. PpOBP was highly expressed in the venom apparatus at the transcriptional and translational levels. PpOBP was located in all parts of venom apparatus including venom gland, venom reservoir, and Dufour's gland. During 0-6 days post adult eclosion, the PpOBP mRNA level peaked at 2 days in the venom apparatus, whereas the protein remained at a high level. In the venom apparatus, the PpOBP mRNA was significantly upregulated following feeding with honey and parasitization. We propose that PpOBP is involved in parasitoid-host interactions.