VEGF-D expression and EGFR mutations in non-small-cell lung carcinoma

Abstract

22149 Background: VEGF-D and EGFR are involved in pathogenesis of lung cancer. The expression of VEGF-D and somatic EGFR mutations are to be investigated. Methods: Sixty-nine primary non-small cell lung cancer and normal lung tissues adjacent to cancer were collected during operation. VEGF-D gene expression level was examined by semi-quantitative SYBR Green real-time RT-PCR with housekeeping gene ACTB as the endogenous control through the establishment of standard curves. For 55 of these patients, RT-PCR products of EGFR spanning exons 18 through 19 and KRAS spanning codons 12, 13, 59 and 61 were directly sequenced. Results: VEGF-D expression level in non-small-cell lung cancer was significantly lower than in normal control (nonparametric Mann-Whitney test, P=0.001), however, there was no difference of VEGF-D expression ratios in normal lung (42.0%) and lung cancer (53.6%). VEGF-D expression level kept no different between lung cancers with and without EGFR or KRAS gene mutation. Total EGFR mutations rati...