Abstract
Purpose: Vascular endothelial growth factor (VEGF) isoforms play different roles in the temporal sprouting of endothelial-lined vessels in a nude mouse peritoneal model as cells respond to nontreated control and gas-plasma-treated bioresorbable poly- d, l-lactide acid 3D scaffolds with human aortic endothelial cells (HAEC). Methods and materials: Nude mice peritoneums were incubated with HAEC (CW=control; TW=gas-plasma treated) or polymer scaffolds (C p=control; T p=treated) for 12, 24 and 72 days. Cytoplasmic and nuclear protein fractions were isolated using NER, electrophoresized using NuPAGE–MES and analyzed by WesternBreeze Chemiluminescent. Results: Prominent VEGF bands included 28, 45 and 62 kDa; 52-kDa VEGF observed in cytoplasmic TW fractions contributed about 18.6% at 12 days, 20.0% at 24 days and 13.1% at 72 days of the total VEGF signal. Yet, it was only noted in CW at 72 days where it accounted for 6.9%. A unique 32-kDa band appeared in both C p (24.6%) and T p (18.3%). Significant differences between band densities occurred for cytoplasmic nuclear CW24–TW24 ( P=.022), CW72–TW72 ( P=.011) and, also, cytoplasmic C p24–T p24 ( P=.038). Conclusions: The temporal and spatial organization of the TW isoforms results in more angiogenesis.
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