Abstract
This study was carried out to detect the favorable mycelial growth conditions and effect of electric pulse stimulation on fruit body production of SC-1 strain of Sparassis crispa. The optimum growth in PDA was found at 25°C (67 mm colony diameter in 28 days) followed by 20°C (63 mm colony diameter in 28 days). There was no mycelial growth at 35°C. The most favourable initial media pH range was found to be 5 - 7 and colony diameter measurements were not statistically different among these pH values (p > 0.05). However, the highest growth was obtained at pH 6 (57 mm colony diameter in 28 days). A basal medium composed of 0.05 g MgSO4, 0.46 g KH2PO4, 1.0 g K2HPO4, 120 μg thiamine-HCl, 20 g agar and 1000 ml of distilled water was used to investigate growth response of S. crispato different carbon and nitrogen sources. In 28 days, fructose and glucose exhibited best growth (49.4 mm and 31.6 mm colony diameters respectively) and there was no growth on the basal medium supplemented with galactose. Basal medium supplemented with glycine and alanine as nitrogen sources resulted in best growth, 54.4 mm and 50.5 mm colony diameters respectively. There was no mycelial growth in culture medium supplemented with ammonium acetate, ammonium phosphate, arginine and histidine. Electric pulse stimulation improved the fruiting body production. The yields obtained from all bottles in which electric pulse was applied were significantly higher than the yields from the bottles in which electric pulse was not applied. The percent increases of fresh weight yield from control on 100, 120, 130, and 170 kilovolts were 36%, 44%, 75% and 81% respectively. As regard to dry weight yield, the percent increases from control on 100, 120, 130, and 170 kilovolts were 27%, 54%, 63% and 67% respectively.
Highlights
Sparassis crispa is an edible mushroom with a number of reported medicinal benefits
Having seen that this technology has worked with success in a number of mushrooms, a study was conducted to investigate the effect of electric pulse stimulation on fruit body development and yield of SC-1 strain of S. crispa
Effect of pH This study shows that slightly acidic to neutral initial agar media pH is most favourable for mycelial growth of S. crispa (Figure 3 and Figure 4)
Summary
Sparassis crispa is an edible mushroom with a number of reported medicinal benefits This fungus is commonly known as cauliflower mushroom because the shape of its fruiting body closely resembles with cauliflower. As reported by Kimura [3], the fruit body of S. crispa has about 90% water and contains 13.4 g protein, 2.0 g fat, 21.5 g carbohydrate, 1.8 g ash and 61.2 g dietary fibre per 100 g of dry weight This fungus belongs to the phylum; Basidiomycota, class; Agaricomycetes, order; Polyporales, family; Sparassidaceae, genus; Sparassis and species; crispa [3]. Islam and Ohga [11] reported a significant increase in fruit body yield of Tricholoma matsutake as a result of applying electric pulse Having seen that this technology has worked with success in a number of mushrooms, a study was conducted to investigate the effect of electric pulse stimulation on fruit body development and yield of SC-1 strain of S. crispa. We carried out experiments to determine the effect of environmental and nutritional factors on mycelial growth of SC-1 strain of S. crispa
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