Abstract
INTRODUCTION: Canine blood banking in veterinary medicine is an expanding market. Once the demand for blood products increased all over the world, canine blood banks have focused attention on the risk of spreading diseases through blood transfused products. The need to preserve a healthy donor-pool, free of blood-borne infectious diseases, mainly in endemic areas, led to the implementation of appropriate protocols for screening canine blood donors using specific tests.OBJECTIVES: The aim of this study was to evaluate the presence of Anaplasma phagocytophilum/Anaplasmaplatys, Echrlichiacanis/Echrlichiaewingii, Dirofilariaimmitis and Borrelia burgdorferi using the enzyme immunoassay technology (EIA) among the donors of a Romanian canine blood bank, from January 2015 to December 2016.METHODS: Blood samples from 575 donors were collected and 1253 tests were performed with SNAP 4DX Plus® (IDEXX Laboratories, Fremont, CA) to reveal the presence of D. immitis antigens and the antibodies toward A. phagocytophilum and/or A. platys, E. canis and/or E. ewingii and B. burgdorferi.RESULTS: The results of this holistic approach show that all blood samples provided negative results for B. burgdorferi and E.canis/E. ewingii (0/1253), while 0.87% (11/1253) samples provided positive results for A. phagocytophilum/A. platys and 6.94% (87/1253) for D. immitis.CONCLUSION: The next studied topic would be to compare the results provided by the EIA technology with results of real time PCR and qPCR regarding these vector-borne pathogens.
Highlights
Canine blood banking in veterinary medicine is an expanding market
The results of this holistic approach show that all blood samples provided negative results for B. burgdorferi and E.canis/E. ewingii (0/1253), while 0.87% (11/1253) samples provided positive results for A. phagocytophilum/A. platys and 6.94% (87/1253) for D. immitis
Between January and December 2015, 352 dogs were selected as potential donors, but only 302 were included in the blood donor registry, while 50 were excluded due to the positive results of the performed immunological tests (46 dogs with Dirofilaria immitis Ag, and 4 dogs with A. phagocytophilum/A. platys Ab) (Table 3)
Summary
Canine blood banking in veterinary medicine is an expanding market. Once the demand for blood products increased all over the world, canine blood banks have focused attention on the risk of spreading diseases through blood transfused products. The approach of blood banking (BB) and transfusional medicine has evolved from an empirically and rare procedure, to a modern and currently available treatment (Greenwalt, 1997; Yagi & Bean, 2016). This trend has been closely connected to the evolution of blood bank equipment and diagnostic methods, making blood transfusions (BT) much safer (Wardrop, 2016). Considering the above mentioned criteria, all blood donors should be tested for various blood-borne pathogens which can potentially cause diseases in the recipient (Wardrop, 2016)
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