Abstract
Additional evidence is presented that the transmission of pea enation mosaic virus (PEMV) by pea aphids, Acyrthosiphon pisum (Harris), from and to sweetpea, Lathyrus odoratus L., is most efficiently done by young nymphs, Apparently virus dosage can be varied by alter. ing the acquisition access period, and such variations affect-(1) the number of insects that transmit; (2) the net transmission rate; and (3), to a lesser extent, the period of time that inoculativity is retained. The total number of plants used in virus retention experiments can be reduced by extending the duration of the transfer interval. If such is done, the data indicate that the value of the mean weighted retention period is not markedly changed, although somewhat biased toward a higher value as the interval is lengthened. A similar bias occurs in the estimate of the net transmission rate.
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