VE-PTP and VE-cadherin ectodomains interact to facilitate regulation of phosphorylation and cell contacts

Abstract

Corrigendum18 August 2005free access VE-PTP and VE-cadherin ectodomains interact to facilitate regulation of phosphorylation and cell contacts Roman Nawroth Roman Nawroth Search for more papers by this author Gregor Poell Gregor Poell Search for more papers by this author Alexander Ranft Alexander Ranft Search for more papers by this author Stephan Kloep Stephan Kloep Search for more papers by this author Ulrike Samulowitz Ulrike Samulowitz Search for more papers by this author Gregor Fachinger Gregor Fachinger Search for more papers by this author Matthew Golding Matthew Golding Search for more papers by this author David T Shima David T Shima Search for more papers by this author Urban Deutsch Urban Deutsch Search for more papers by this author Dietmar Vestweber Dietmar Vestweber Search for more papers by this author Roman Nawroth Roman Nawroth Search for more papers by this author Gregor Poell Gregor Poell Search for more papers by this author Alexander Ranft Alexander Ranft Search for more papers by this author Stephan Kloep Stephan Kloep Search for more papers by this author Ulrike Samulowitz Ulrike Samulowitz Search for more papers by this author Gregor Fachinger Gregor Fachinger Search for more papers by this author Matthew Golding Matthew Golding Search for more papers by this author David T Shima David T Shima Search for more papers by this author Urban Deutsch Urban Deutsch Search for more papers by this author Dietmar Vestweber Dietmar Vestweber Search for more papers by this author Author Information Roman Nawroth, Gregor Poell, Alexander Ranft, Stephan Kloep, Ulrike Samulowitz, Gregor Fachinger, Matthew Golding, David T Shima, Urban Deutsch and Dietmar Vestweber The EMBO Journal (2005)24:3158-3158https://doi.org/10.1038/sj.emboj.7600806 This article corrects the following: VE-PTP and VE-cadherin ectodomains interact to facilitate regulation of phosphorylation and cell contacts16 September 2002 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Correction to: The EMBO Journal (2002) 21, 4885–4895. doi:10.1093/emboj/cdf497 In the above paper, the induction of the mutant VE-PTP R/A in triple-transfected CHO cells expressing VE-cadherin and Flk (clone RA-7) increased the function of VE-cadherin similarly, as did induction of the wild-type form of VE-PTP (Figure 9D). We have found now that clone RA-7 had erroneously been transfected with wt VE-PTP instead of the mutant form. Analysing new triple transfectants expressing VE-PTP R/A revealed that this mutant did not stimulate the adhesive activity and did not affect phosphorylation of VE-cadherin, as shown in the figure. We wish to stress that the major findings of the paper (association of VE-PTP and VE-cadherin as well as enhancement of VE-cadherin adhesive function by VE-PTP) are not affected by this mistake. Figure 1.VE-PTP, but not VE-PTP-R/A, reduces paracellular permeability in VE-cadherin/Flk-1/VE-PTP triple-transfected CHO and inhibits tyrosine phosphorylation of VE-cadherin. Paracellular permeability for FITC-dextran was analysed with monolayers of various stably transfected CHO cells. Left graph: Analysis of triple-transfected CHO-F12, transfected with VE-cadherin, VEGFR-2 and mifepristone-inducible FLAG-VE-PTP. Stimulation with mifepristone for 10 h resulted in a 4574% reduction of cell permeability (black) as compared to mockstimulated cells (light grey). Right graph: Analysis of triple-transfected CHO-RA25.5, transfected with VE-cadherin, VEGFR-2 and mifepristoneinducible FLAG-VE-PTP-R/A. Stimulation with mifepristone for 10 h resulted in no significant reduction of cell permeability (black) as compared to mock-stimulated cells (light grey). Upper panel: CHO-F12 and CHO-RA25.5 cells, either induced (+) or not induced (−) with mifepristone were analysed by immunoprecipitations with anti VE-cadherin antibodies, followed by immunoblotting with an antibody against phospho-tyrosine (anti-PY). The same filter was reprobed with an antibody against VE-cadherin, demonstrating equal loading of VE-cadherin. Download figure Download PowerPoint The authors would like to apologize for their mistake. Next ArticlePrevious Article Read MoreAbout the coverClose modalView large imageVolume 24,Issue 17,September 7, 2005Organic Forms by Simon Walter. The idea for this drawing was based on images of fractals and roots. Inspiration came from surrealist paintings, comics, and weird scientists, says Simon. Contact the artist at [email protected] Volume 24Issue 177 September 2005In this issue FiguresRelatedDetailsLoading ...