VDR gene polymorphism in children with allergic diseases

Abstract

Background. To investigate the frequency of vitamin receptor gene D (VDR) polymorphism in children with allergic diseases. Materials and methods. We enrolled 130 children (66 boys and 64 girls) with allergic diseases aged from 1,5 to 16 years old. All children underwent clinical and allergological examination. 93 children (71,5%) had isolated allergy, mainly food allergy and atopic dermatitis, 37 children (28,4%) had bronchial asthma, associated with allergic rhinitis and urticaria. The control group included 41 healthy children aged from 1 to 10 years old. For DNA allotment the kit ProbaRapid Genetics of DNA Technology (Russia) was used. Analysis of polymorphic marker FokI (rs 2228570), BsmI (rs 1544410) and TaqI (rs 731236) of VDR gene was performed by PCR in real time using the detecting thermocycler DT-96, and kits for DNA diagnostics company DNA technology (Russia). Research metabolite 25-OH (25-hydroxyvitamin D2 and D3) of vitamin D was carried out by ELISA commercial kit DIAsource (Belgium). Results. Significantly increased frequency of allele A in the VDR gene BsmI site (OR=1,81, p=0,04) and homozygous A/A, heterozygous G/A genotype (OR=2,03, p=0,05 and OR=1,8, p=0,05, respectively) was found, that enables to consider it as a prognostic marker associated with the development of allergic disease risk. Statistically significant reduction in the concentration of 25-OH-vitamin D (p=0,02) in children with the heterozygous variant A/G and homozygous G/G in Fok1 VDR gene was found. Conclusion. There were significant differences in frequencies of genotypes and alleles of DVR gene in children with allergic diseases in the population. These results create the preconditions for the development of new methods of prevention of vitamin D deficiency, taking into account the individual characteristics of its metabolism.