Vaspin promotes the osteogenic differentiation of bone marrow mesenchymal stem cells in rats through the Wnt/β-catenin signaling pathway

Abstract

Objective To observe the effect of vaspin on the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells in rats and its potential mechanism. Methods Bone marrow mesenchymal stem cells(BMSCs)from 4 weeks aged rats were isolated and cultured. The BMSCs were treated with osteogenic induction medium and different concentrations of vaspin and Wnt signaling pathway inhibitor DKK1. The proliferation was detected by CCK8 method at 24, 48, 72 h. After 7 days, the mRNA expressions of ALP, Runx2, β-catenin were detected by realtime qPCR. The expression levels of Runx2 and β-catenin protein were detected by Western blot. After 21 days, alizarin red stained mineralized nodules and quantitative detection were performed. Results Vaspin had no effect on the proliferation but promoted the expression of osteogenic differentiation gene ALP, Runx2, and also increased β-catenin mRNA and expression of Runx2 and β-catenin protein. Mineralized nodules were brown and increased, OD value of vaspin group was higher than control group. After adding the DKK1, the expression of ALP, Runx2, β-catenin mRNA and Runx2, β-catenin protein were significantly decreased(all P<0.05). Conclusion Vaspin can promote BMSCs into osteogenic differention through the Wnt/β-catenin signaling pathway. (Chin J Endocrinol Metab, 2016, 32: 505-509) Key words: Vaspin; Bone marrow mesenchymal stem cells; Wnt/β-catenin; Osteogenic differentiation