Vasorelaxant study of standardized extract of Pericarpium Citrus reticulatae

Abstract

Event Abstract Back to Event Vasorelaxant study of standardized extract of Pericarpium Citrus reticulatae CHU SHAN TAN1 and MUN FEI YAM1* 1 School of Pharmaceutical Sciences, University of Science Malaysia, Malaysia Background Pericarpium Citrus reticulatae (Chen pi in Chinese) was widely used as an important ingredient in the prescription of Traditional Chinese Medicine (TCM) to treat hypertension and is one of the famous ingredients officially listed in Chinese Pharmacopoeia. Since Chen pi has been included in major TCM formulations to treat hypertension, it is a correlated and justifiable expectation that Chen pi possesses vasorelaxant properties. Methods This study is designed to investigate the vasorelaxant effect of Chen pi standardized extracts using Sprague Dawley rat isolated thoracic aortic ring assay. Results Among the different solvent extracts, water extract of Chen pi (CRW) exhibited the strongest vasorelaxant activity. CRW caused the relaxation of the phenylephrine pre-contracted aortic rings in the presence and absence of endothelium as well as in potassium chloride pre-contracted endothelium-intact aortic ring. The incubation of propranolol (β-adrenergic receptor blocker), atropine (muscarinic receptor blocker), Nω-nitro-L-arginine methyl ester (NO synthase inhibitor), ODQ (sGC inhibitor), indomethacin (COX inhibitor), 4-aminopyridine (KV blocker), barium chloride (Kir blocker), and glibenclamide (KATP blocker) significantly reduced the vasorelaxant effects of CRW. CRW was also found to be active in reducing calcium releases from the sarcoplasmic reticulum and suppressing the voltage-operated calcium channels. Conclusion The vasorelaxant effect of CRW on rat aorta involves NO/sGC, calcium and potassium channels, muscarinic and β-adrenergic receptors. Keywords: Pericarpium Citrus reticulatae, NO/sGC pathway, Potassium and calcium channels, Traditional Chinese Medicine, Vasorelaxation Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Oral Presentation Topic: Metabolic diseases Citation: TAN C and YAM M (2019). Vasorelaxant study of standardized extract of Pericarpium Citrus reticulatae. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00067 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. MUN FEI YAM, School of Pharmaceutical Sciences, University of Science Malaysia, Penang, Malaysia, yammunfei@yahoo.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers CHU SHAN TAN MUN FEI YAM Google CHU SHAN TAN MUN FEI YAM Google Scholar CHU SHAN TAN MUN FEI YAM PubMed CHU SHAN TAN MUN FEI YAM Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.