Abstract
Objectlve To study the ability to repair large segmental bone detects of vasculanzed bone of tissue engineering constructed by co-seeding mesenchymal stem cells(MSCs)and endothelial cells (ECs)induced from the MSCs in porous β-tricalcium phosphate(TCP).Methods Rabbit MSCs were obtained,cuhured,and induced to differentiate into ECs. 1.5 cm ulna diaphyseal defects were made bilaterally in 32 rabbits.The 64 bone defects were randomly divided into 4 equal groups Group A:without treatment,Group B,implanted with β-TCP,Group C,implanted with tissue-engineered bone(MSCs+β-TCP),and Group D,implanted with vascularized bone of tissue engineering(MSCs+ECs+β-TCP).Xray and ECT examination,HE staining of tissue slices of the implants,and biomechanical test of the specimens were carried out 4,8,12,and 16 weeks postoperatively to evaluate the repairing effect of the bone defects and vascularization of the implants.Results The bone defects of Group A showed little trace of osteogenesis.The bone defects of Group D showed a more perfect repairing effect and vasculanzation of implants than Group C.Conclusion The vascularized bone of tissue engineering constructed by MSCs and ECs induced from MSCs co-seeded in porous β-TCP can accelerate the vascularization and osteogenesis process of the implants,and is more effective in repairing large segmental bone defects. Key words: Stem cells; Tissue therapy; Osteogenesis
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
