Vascular endothelial growth factor genotypes and haplotypes contribute to the susceptibility of obstructive sleep apnea syndrome.

Chao Cao,Zhe Dong,Zaichun Deng,Dan Lv,Qunli Ding,Huahao Shen,Zhongbo Chen,Shifang Sun,Thomas Penzel

doi:10.1371/journal.pone.0114582

Abstract

BackgroundTo investigate whether VEGF polymorphisms (-460T/C, +405G/C, and +936C/T)/haplotypes influence the susceptibility of obstructive sleep apnea (OSA).MethodA prospective case-control study was conducted to evaluate the genetic effects of VEGF polymorphisms on the development of OSA. 150 patients and 225 healthy controls were recruited for this study and their genotypes were determined by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The odds ratios (OR) and 95% confidence intervals (CI) were calculated by logistic regression analysis.ResultOur study showed that the -460C allele (C vs. T: OR = 1.95, 95% CI = 1.38–2.76) and +936T allele (T vs. C: OR = 1.48, 95% CI = 1.02–2.15) were associated with an increased OSA risk, whereas +405C allele was associated with a decreased susceptibility to OSA (C vs. G: OR = 0.61, 95% CI = 0.45–0.83). Compared with the most common haplotype CCT, CGC (OR = 2.22, 95% CI = 1.19–4.13) and TGC (OR = 3.83, 95% CI = 1.56–9.40) were associated with a significantly increased risk of OSA.ConclusionThese observations implied that VEGF gene polymorphisms might be associated with the susceptibility to OSA. These results need to be validated by other independent studies, especially in diverse ethnic populations.

Highlights

Obstructive sleep apnea (OSA) syndrome is a respiratory disorder characterized by a unique form of intermittent hypoxia, with short, repetitive cycles of hypoxia and reoxygenation [1]
A prospective case-control study was conducted to evaluate the genetic effects of vascular endothelial growth factor (VEGF) polymorphisms on the development of OSA. 150 patients and 225 healthy controls were recruited for this study and their genotypes were determined by polymerase chain reaction and restriction fragment length polymorphism (PCRRFLP)
Result: Our study showed that the -460C allele (C vs. T: OR51.95, 95% CI51.38– 2.76) and +936T allele (T vs. C: OR51.48, 95% CI51.02–2.15) were associated with an increased OSA risk, whereas +405C allele was associated with a decreased susceptibility to OSA (C vs. G: OR50.61, 95% CI50.45–0.83)

Summary

Introduction

Obstructive sleep apnea (OSA) syndrome is a respiratory disorder characterized by a unique form of intermittent hypoxia, with short, repetitive cycles of hypoxia and reoxygenation [1]. Hypoxia is a major stimulator of vascular endothelial growth factor (VEGF) expression [3]. The common three VEGF SNPs, -460T/C (rs833061) and +405G/C (rs2010963) in the 59-untranslated region and +936C/T (rs3025039) in the 39untranslated region, were found to be associated with differential VEGF expression and involved in many kinds of disorders in which angiogenesis was critical in the development of disease [12], [13]. To investigate whether VEGF polymorphisms (-460T/C, +405G/C, and +936C/T)/haplotypes influence the susceptibility of obstructive sleep apnea (OSA). Conclusion: These observations implied that VEGF gene polymorphisms might be associated with the susceptibility to OSA. These results need to be validated by other independent studies, especially in diverse ethnic populations

Methods

Results

Conclusion