Vascular Connections Into the Grape Berry: The Link of Structural Investment to Seededness.

Zeyu Xiao,Suzy Y Rogiers,Leigh M Schmidtke,Stephen D Tyerman,Rosemary G White,Aude M Gourieroux,Vinay Pagay,Sabrina Chin

doi:10.3389/fpls.2021.662433

Abstract

Vascular bundles in the grape pedicel and berry contain the conduits, phloem and xylem, for transport of water, sugar, nutrients and signals into and through the grape berry and play a critical role in berry growth and composition. Here, we assess the vascular anatomy within the proximal region of the berry. Guided using a 3D berry model generated by micro-CT, differential staining of transverse sections of berries and receptacles was followed by fluorescent microscopy. Morphometric and vascular characteristics were analyzed within the central proximal region (brush zone, a fibrous extension from the pedicel vascular system into the berry) of the seeded cultivars Shiraz and Sauvignon Blanc, as well as the stenospermocarpic cultivars Ruby Seedless and Flame Seedless. Observations revealed a change in vascular arrangement from the receptacle into the berry brush zone and differences in xylem element size as well as xylem and phloem area relationships. Xylem anatomical and derived hydraulic parameters, as well as total tissue area of xylem and phloem varied between cultivars and in receptacle and berry components. Variation in vascular growth between grape pedicels and berries was independent of seededness. Differences in receptacle xylem vessel size and distribution could contribute to cultivar-dependent xylem backflow constraint.

Highlights

Growth of fleshy fruits such as the grape (Vitis vinifera) depends on the coordination between vascular transport and cell expansion within the fruit (Matthews and Shackel, 2005)
The vascular connection between the pedicel and the grape berry has been characterized in commercial grape cultivars
Xylem anatomical and derived hydraulic parameters as well as total vascular tissue area were found to vary between cultivars and in receptacle/berry components, which could result in different K between cultivars and affect water retention of the berries

Summary

Introduction

Growth of fleshy fruits such as the grape (Vitis vinifera) depends on the coordination between vascular transport and cell expansion within the fruit (Matthews and Shackel, 2005). Grapevine vascular bundles transport water, nutrients and photo-assimilates (Rogiers et al, 2001, 2006b; Tilbrook and Tyerman, 2009; Keller et al, 2015; Knipfer et al, 2015) and play a critical role in berry growth and composition. Vascular bundles are composed of xylem, phloem and meristematic tissues (vascular cambium) (Sachs, 1981). Differentiation from the cambium results in addition of xylem and phloem both inwards and outwards (Jouannet et al, 2015). The differentiation and growth of the vascular system are hormonally controlled with both environmental and genetic cues that regulate cambium activity and formation (Aloni, 1987; Scarpella and Meijer, 2004)

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