Abstract
Three sub-group lipases of Burkholderia cepacia lipase (BCL), Rhizomucor miehei lipase (RML), and Candida rugosa lipase (CRL) were covalently immobilized on dendrimer functionalized magnetic carbon nanotube and used as catalysts to catalyze biodiesel production. The effects of imprinting molecule, organic solvent, water, methanol, temperature and time interval of methanol addition on the yield of biodiesel were optimized. The results showed that bioimprinting could greatly enhance catalytic performances of the three immobilized lipases. The obtained lipases were then employed to catalyze biodiesel production, and the achieved optimum conditions were: for BCL, water content 5 wt. %, reaction temperature 35°C, and with t-butanol as reaction medium, methanol : oil molar ratio 4:1, its highest biodiesel yield attained 96.4%; for RML, water content 10 wt. %, reaction temperature 50 °C, n-octane as the reaction medium, methanol : oil molar ratio of 5:1, the utmost biodiesel conversion rate was up to 96.2%; and ...
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