Abstract
In the present study, 14 rice specific sequence tagged microsatellite site markers (STMS) were used to developfingerprints and to determine the genetic relatedness among 48 rice genotypes/cultivars. Ten of the 14 SSR primerpairs were found to be polymorphic, generating a total of 39 distinct reproducible alleles with an average of 2.79alleles per primer pair and 22 (56.41%) of the 39 bands amplified emerged as polymorphic. The number of alleles perprimer ranged from one to six. The polymorphic information content (PIC) value ranged from 0.03 to 0.7 with anaverage of 0.35. Cluster analysis based on Jaccard’s similarity coefficient using unweighted pair group methods forarithmetic mean (UPGMA) grouped the 48 rice cultivars into five major clusters with an average similarity index of0.87. A diagrammatic representation of DNA fingerprints of the rice cultivars based on 10 informative SSR markerswas developed for ready reference, which are expected to be key in varietal identification as well as provide IPR tohalt biopiracy.
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