Varietal Diversity of Seed Glucosinolates Content and its Composition in Japanese Winter Rape (Brassica napus L.).

Abstract

To identify varietal diverity of seed glucosinolates content in Japanese rape (Brassica napus L.), total amount and composition of seed glucosinolates content were analysed on 65 Japanese rape and 11 European and North American low glucosinolates rape (Table 1) by means of Palladium-glucosinolate complex method and high-performance liquid chromatography (HPLC) method. The average of total amount of glucosinolates of Japanese cultivars varied from 60. 5 μmol/g of Norin 18 to 161. 4 1lmol/g of Norin 12 (Fig. 1) . In 50 Japanese cultivars, Norin 18 (60. 5 pmol/g) and Norin 16 (65. 4 μmol/g) showed the lowest glucosinolates contents. On the other hand, glucosinolates content of Norin 12 (161.4μImol/g) and Isuzunatane (150.9 μmol/g) were the highest. These four cultivars were originated from interspecific crossing of B. napus X B. campestris. HPLC analysis of seed glucosinolates revealed that rapeseed had 10 main peaks (Fig. 2) . In these peaks, 9 glucosinalates were identified by high-performance liquid chromatography/mass spectrometry. Low gluco-sinolates contents in Norin 18 and Norin 16 resulted from the reduction of Progoitrin and Gluconapin which are Aliphatic glucosinolate. Though, Japanese cultivars have been bred by three different breeding methods, e. g. pure line selection, varietal crossing (B. napus X B. napus) and interspecific crossing (B. napus X B. campestris), each glucosinolate content was not related to the breeding method (Table 2)