Abstract
The effects of cold stress on protein phosphorylation in leaves of rice ( Oryza sativa) seedlings were investigated. Two-week-old rice seedlings were exposed to low temperature (5°) for 6 hr at a relative humidity of 100%. The leaf protein extracts were phosphorylated in vitro. Cold stress stimulated the phosphorylation of a 60 kDa protein in the cold-sensitive rice variety, IR36. In the cold-tolerant rice variety, Kitaibuki, this protein had already been phosphorylated. For greater clarificating of these changes, the effects of cold on rice varieties differing in cold susceptibility were examined. Cold-sensitive rice varieties showed similar the protein phosphorylation in contrast to the cold-tolerant rice varieties. © 1997 Elsevier Science Ltd. All rights reserved
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