Variations of Flavone Glycosides Profile Underscore the Necessity of Quality Control of Prepared Microctis Folium Slices.

Kun-Ping Li,Jiao Guo,Tian-Ling Pan,Min Yuan,Ze-Yu Liang,Chang-Le Liu,Li Zhang

doi:10.1155/2020/8887489

Abstract

Microctis Folium (MF), the dried leaves of Microcos paniculata, is widely used as a medical and food dual-purpose herb in South-east Asia and China. However, the quality control of MF is not well studied. A simple and reliable quality control method was urgently needed for its growing usage. Herein, at first, its main active components were identified by UPLC-ESI-qTOF-MS, and a representative MF flavone glycosides profile consisting of ten compounds was illustrated, which is the most detailed one up to now. Successively, using vitexin as the reference substance, a novel QAMS method with HPLC for quantification of the ten identified flavone glycosides was developed and methodologically validated. Furthermore, making use of the abovementioned QAMS method, quantitative profiling of 21 batches of prepared MF slices collected from different hospital pharmacies were performed. As a result, the total contents of ten flavone glucosides and the content of specific compound showed obvious variations. Using the ten compounds' contents dataset, the 21 batches of samples were divided into two distinct clusters by HCA. In sum, our results indicated that it was of great importance to take quality control of prepared MF slices and we presented a robust and simple method for their quantitative determination, which should be beneficial for the quality control of MF and its derived products.

Highlights

M. paniculata, known as Grewia nervosa, is a medicinal and edible plant widely distributed in South China, Indian, Indonesia, Myanmar, and so on [1]
As discovered in many other plants, the flavone glycosides in Microctis Folium (MF) were presented as C- or O-glycosides (Figure 1). e ten flavone glycosides were all hydroxylated in positions 5 and 7
All these results indicated that the contents of flavone glycosides caused differences in samples of MF from various clinical pharmacies. e chemical variation may be caused by a lot of factors, such as their genetic origin, growing environment, time to collection, process of production, and storage condition

Summary

Introduction

M. paniculata, known as Grewia nervosa, is a medicinal and edible plant widely distributed in South China, Indian, Indonesia, Myanmar, and so on [1]. It has been reported that the constituents of the roots, stems, barks, and fruits of M. paniculata possess α-glucosidase inhibitory, free-radical scavenging, antinociceptive, antidiarrheal, anti-inflammatory, analgesic, and antipyretic activities [2,3,4,5]. MF is described to have positive effects on fever, jaundice, heat-stroke, indigestion, and diarrhea [6]. It is generally used for producing some Chinese patent drugs and some kinds of Chinese herbal tea beverage which are as popular as cola in Western countries. Phytochemical studies show that MF has triterpenoids, alkaloids [11], and, by virtue of its high content of flavonoids, ethanol extract of MF possessing excellent antioxidant and anti-inflammation activities [12,13,14]. Knowledge on its active components qualitative and quantitative analysis is lacking, and the quality control of prepared MF slices is not well investigated, which limits its further development and utilization as functional foods

Methods

Results

Conclusion