Variations of biogenic amine levels in the brain of Pieris brassicae pupae during nondiapausing and diapausing development

Abstract

AbstractThe post‐embryonic development of Pieris brassicae can either be continuous (under a long photoperiod) or interrupted at the pupal stage (induced by a short photoperiod); this phenomenon is termed facultative diapause. Several studies have indicated that certain brain mechanisms could be directly involved in the perception of variations in the photoperiod and could mediate some physiological effects particular to dormancy. Biogenic amines have been particularly implicated in the response to photoperiod variations and also in the regulation of development, especially in diapause induction and termination.High performance liquid chromatography with dual electrochemical detection has therefore been used to measure several biogenic amines in pupal nervous tissues at various stages of nondiapausing and diapausing development.During direct development, the levels of dopamine (DA) and N‐acetyldopamine (NADA: a DA metabolite) in brain were relatively high in 3‐day‐old pupae and at the end of pupal life (on the 8th day). Dihydroxyphenylacetic acid (another metabolite of DA) showed no variation. Serotonin was mainly observed in 2–3‐day‐old pupae but 5‐hydroxyindoleacetic acid was never detected. In young diapausing insects, similar variations of DA levels were observed even though a slight decrease of DA metabolites was noted. Serotonin appeared somewhat later (4–5 days) and attained higher levels. In late diapausing pupae, a marked increase in DA levels was observed, especially when pupae were kept at low temperature (4°C). During diapause, serotonin levels were reduced or even absent.