Variations in time and space of an Andean wild population of T. infestans at a microgeographic scale

Abstract

BackgroundWild populations of Triatoma infestans are now believed to be the source of reinfestation of dwellings in some Andean areas and could impede the full achievement of vector control campaigns in this region. Given the poor knowledge of these populations in natural conditions, their basic biology traits, such as monthly demographic variations and movements of individuals, were explored.MethodsA previously identified wild population of T. infestans in a field adjacent to a group of isolated houses in an Andean valley (department of La Paz, Bolivia) was explored using regular capture assays over 13 months in 50 sites selected at the beginning of the study. The capture-mark-recapture method was applied monthly using mouse-baited adhesive traps for captures and fingernail polish of different colors for the marking.ResultsThe monthly capture assays did not show significant differences between rainy and dry seasons, showing evidence for a certain stability of the wild T. infestans population with only the nymph population tending to decline during the middle of the rainy season when rain is more intensive. Throughout the study, the monthly average number of bugs was 51.1 ± 25.3 per assay, 91.1% were nymphs, and they were found at 30 of the 50 sites (60%). The number of times a site was positive varied from one to 13. Site infestation was associated with the underground position of the traps, and rocks around and in the surroundings of the traps. The recaptures after marking were successful (138 recaptures over the study). The marking made it possible to detect for 14.5% of the recaptures significant movements of adults (up to 168 m) and nymphs (up to 34 m). Some bugs (nymphs and females) were recaptured after 5 months. For adults, recaptures (46 in total) mostly occurred between September and March. Females were recaptured twice as frequently as males.ConclusionThe Andean wild populations of T. infestans showed a strong spatial and temporal stability during the year-long study. Dispersal may occur mainly during the rainy season. The capture-mark-recapture method was successful and the longevity of the bugs and the distances covered by nymphs and adults were recorded.