Abstract
The intracellular potential of human peripheral blood lymphocytes and monocytes was measured with a modified neurophysiological system and was found to vary between −20 mV and +20 mV. By depleting the total mononuclear fraction of monocytes and B-lymphocytes, and by using various separation procedures to derive monocyte, B-lymphocyte-, T-lymphocyte-, and null cell-enriched populations, it was possible to show that monocytes and the majority of the B-lymphocytes had positive intracellular potentials, whereas T-lymphocytes and null cells had negative intracellular potentials. Various factors, which include in vitro culturing, PHA and Con A stimulation, and possibly maturity, significantly affect the intracellular potential.
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