Variations in the Axon Reaction after Different Types of Nerve Lesion

Abstract

The retrograde nerve cell reaction was studied after evulsion, transection and crush lesion of the facial nerve in rats. Crush lesion caused barely discernible light and electron microscopic changes. The Nissl bodies became slightly smaller than normal and the arrangement of the granular endoplasmic reticulum (rER) somewhat more irregular. The crush lesions were followed by complete functional and morphologic recovery. After nerve evulsion, the cells showed severe chromatolysis, nuclear caps, nuclear eccentricity, and folding of the nuclear membrane. Ultrastructurally there was a dispersion of the rER and formation of laminated dense bodies. Lager, the rER was partly degranulated and some of the polyribosomes dissociated. These neurons ultimately disappeared. Transection of the nerve caused an intermediate axon reaction and a moderate loss of neurons. It is concluded that certain neurons may regenerate after axotomy in spite of minimal light and electron microscopic changes in the nerve cell bodies, and that the same neurons may show the typical axon reaction after more severe nerve injuries. Mechanisms which may be involved in the regulation of the retrograde nerve cell reaction after axotomy are briefly discussed.