Abstract
Abstract Three gibberellins; GA3, GA4, GA7; and abscisic acid (ABA) from the shoot tips of greenhouse grown ‘Gloria’ azaleas, Rhododendron sp.L., were tentatively identified using column chromatography, gas-liquid chromatography, and Rumex leaf senesence bioassay. Growth regulators were quantitatively estimated biweekly from 6 weeks after shoot tip removal until anthesis. GA3 levels remained nominal for the normal commercial treatment until after plants were returned to 19°C from the 9° cooler. Endogenous GA3 levels then peaked at 0.6 μg/bud at anthesis. GA4 levels remained fairly constant for all treatments and times at 0.1 μg/bud. GA7 levels remained fairly constant below 2 μg/bud except in the cold-treated plants when endogenous GA7 levels peaked at 0.6 μg/bud at the time that plants were removed from the cooler. ABA levels were similar until 24 wks. from pinch when the levels dropped to undetectable levels in cold-treated plants and increased in treatments not given a cold treatment by peaking at 0.1 μg/bud at 28 wks. from pinch. Of the commercially available gibberellins that were monitored, GA7 seemed to be the best treatment for chemically overcoming flower bud dormancy in azalea.
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