Abstract
Kinetics of alkaline phosphatase in sediments of a shallow Chinese freshwater lake (Lake Donghu) were investigated. Spatially, among 20 sites sampled, V max and K m values of alkaline phosphatase in surface sediments were higher in the zone adjacent to sites with the highest chlorophyll a concentrations. Vertically, there was a peak in V max at intermediate sediment depths in addition to the expected maximum at the surface. Some inhibitors, such as CuSO4, ZnSO4 and Na2WO4, showed significantly different effects on kinetics of alkaline phosphatase in interstitial water and sediments. Moreover, alkaline phosphatase in interstitial water and sediments responded to Na2WO4 in different ways in Lake Taihu. These observations imply that the enzyme is immobilized in sediments, which became more stable with accelerated eutrophication, as suggested by highest alkaline phosphatase activity (APA) in sediments corresponding with highest water column chlorophyll a concentrations in Lake Donghu.
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