Variations in fluid chemistry and membrane phospholipid fatty acid composition of the bacterial community in a cold storage groundwater system during clogging events

Abstract

Abstract In order to monitor the operating mode of the cold storage of the German Parliament (North German Basin) the fluid chemistry and the phospholipid fatty acid (PLFA) composition of the indigenous microbial community have been monitored from August 2006 to August 2009. During this time two periods of reduced injection (clogging events) characterized by Fe precipitates and microbial biofilms in filters occurred in the injection wells impairing the operating state of the investigated cold storage. The fluid monitoring revealed the presence of sufficient amounts of potential C and energy sources (e.g. DOC and SO 4 2 - ) in the process water to sustain microbial life in the cold storage. In times of reduced injection the PLFA inventory of the microbial community differs significantly from times of normal operating phases indicating compositional changes in the indigenous microbial ecosystem. The most affected fatty acids (FAs) are 16:1ω7c (increase), 16:1ω7t (decrease) and 18:1ω7c (increase), interpreted to originate mainly from Fe and S oxidizers, as well as branched FA with 15, 16 and 17 C atoms (decrease) most likely representing sulfate-reducing bacteria (SRB). Based on this variability, PLFA ratios have been created to reflect the increasing dominance of biofilm forming S and Fe oxidizers during the disturbance periods. These ratios are potential diagnostic tools to assess the microbiological contribution to the clogging events and to find appropriate counteractive measures (e.g. mechanical cleaning vs disinfection). The correlation between changes in the PLFA composition and the operational state suggests that microbially mediated processes play a significant role in the observed clogging events in the investigated cold storage.