Abstract
The effect of mitomycin C on two human ovarian cancer cell lines was measured during several exposure times and concentrations using the Human Tumour Colony-forming Assay (HTCA). Changes in exposure time and concentration resulted in considerable differences in tumour cell survival. It is concluded that several exposure times and concentrations are necessary for in vitro sensitivity testing. We suggest alternative criteria derived from pharmacokinetic data in patients instead of one-tenth of the peak plasma level which is the usual practice.
Highlights
In vivo drug behaviour can be characterized by pharmacokinetic parameters such as peak plasma concentration, concentration-time product, elimination half life, clearance and volume(s) of distribution which, for the same drug may vary considerably between patients (Slee et al, 1983)
The material was centrifuged for 5 min at 600 rpm, and washed in Dulbecco's MEM and 10% newborn calf serum (NBCS)
Sensitivity data For both cell lines the influence of the exposure time and concentration of MMC on the tumour cell survival in the Human Tumour Colony-forming Assay (HTCA) is depicted in figure 1
Summary
Two cell lines denoted as C-Ov-362 and C-Ov-318 were derived from fresh specimens of pleural and ascites aspirates from ovarian cancer patients. A few colonies grown in the HTCA were transferred from a Petri dish to a monolayer culture; both cell lines were tested between the 15th and 20th passage. The monolayer cultures were treated with a 0.1% trypsin solution. The material was centrifuged for 5 min at 600 rpm, and washed in Dulbecco's MEM and 10% newborn calf serum (NBCS). The cell suspension was pushed through 21, 23 and 25 gauge needles.
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