Variations in biochemical characteristics of Zataria multiflora in response to foliar application of zinc nano complex formed on pomace extract of Punica granatum

Abstract

The purpose of this study was to see how green synthesized zinc nanostructure (Zn NS) applied to the leaves of Zataria multiflora Boiss. affected the plant’s essential oil constituents, antioxidant activity, phenolic compounds, total phenolic, flavonoid, carotenoid, chlorophyll and anthocyanin contents. A completely randomized block design was used in this experiment. The zinc chelate (Zn-EDTA) was compared to foliar application of Zn NS fertilizer at three dosages (0, 100, and 300 mgL−1). There was no significant difference on Zn content between Zn NS fertilizer with pomace extract at 100 and 300 mg L−1 concentrations. In contrast to Zn-EDTA and control plants, fertilization with Zn nano-fertilizer at 300 mgL−1 resulted in substantial essential oil production and major constituent of linalool. As shown in the findings, various sources of zinc fertilizer considerably increased oxygenated monoterpenes content. Other analyzed phytochemical attributes changed significantly with Zn nano-fertilizer, with the maximum level of evaluated phytochemical parameters observed at a dosage of 300 mgL−1 when compared to the untreated reference. Furthermore, we discovered a favorable relationship between phytochemical characteristics. According to the findings of this study, green synthesized Zn NS has a substantial impact on all biochemical parameters. It may be inferred that Zn NS must be applied to the leaves of Z. multiflora to improve quality and quantity.