Abstract

Ecdysteroid titer in whole extracts of female O. gammarellus varies during the course of the moult cycle: it is minimal at the beginning of the intermoult cycle (stages A, B and Ca) and in D0, and maximal in D2. In hemolymph, the hormone content presents a single peak level in D2; this peak is constituted by ecdysterone, as revealed by HPLC. Ovaries, at the end of vitellogenesis, contain ecdysterone and low polarity substances. Ecdysteroids are also present in eggs after laying. Variations in ecdysteroid levels seem to influence oocyte growth; vitellogenin synthesis, however, is independent of hormonal fluctuations.

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