VARIATION OF THE METABOLIC PRODUCTION OF THE ENDOPHYTIC FUNGUS DIAPORTHE SP. OF CLITORIA GUIANENSIS BENTH USING OSMAC

Abstract

The increasing number of sequenced genomes of fungi has revealed a large number of biosynthetic clusters of secondary metabolites that are still unknown, which reveals the real and vast biosynthetic capacity not yet explored and allows to understand new routes of synthesis, substances and biological properties in silenced genetic clusters. The objective of this work was to detect new natural products from the cultivation of microorganisms under different fermentation conditions using strategies as OSMAC. Three crude extracts (A, B and C) obtained from the endophytic fungus Diaporthe sp. isolated from Clitoria guianensis, were submitted to HPLC-DAD and 1H-NMR analyses. The analyzes showed differences in the production of secondary metabolites in the different culture media. Extract C led to a higher number of secondary metabolites. The crude extracts were submitted to antioxidant and allelochemical activity, the three crude extracts presented positive activity for both assays. Flavones, flavonols and xanthones were identified in all crude extracts, alkaloids in extracts A and C, and steroids and triterpenoids in extract C.