Abstract
Escherichia coli was continuously cultivated in a disc turbine agitated laboratory fermenter at constant dilution rate under conditions of carbon limitation. Agitation rate (impeller speed) was varied over the range 600 to 1500 rev. min-1. As previously reported, the mean cell volume was found to increase linearly with increase in agitation rate, whereas total cell counts and dry cell weights remained constant. Measurements of intracellular sodium and potassium concentration showed that these both increased as the cell volume increased: the potassium content was about twenty times the sodium content and the intracellular content of each ion doubled over the range of agitation rates tested.
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