Abstract
The objective of the current study was to investigate (i) the outcome of experimentally induced Escherichia coli mastitis in primiparous cows during early lactation in relation with production of eicosanoids and inflammatory indicators, and (ii) the validity of thermography to evaluate temperature changes on udder skin surface after experimentally induced E. coli mastitis. Nine primiparous Holstein Friesian cows were inoculated 24 ± 6 days (d) after parturition in both left quarters with E. coli P4 serotype O32:H37. Blood and milk samples were collected before and after challenge with E. coli. The infrared images were taken from the caudal view of the udder following challenge with E. coli. No relationship was detected between severity of mastitis and changes of thromboxane B2 (TXB2), leukotriene B4 (LTB4) and lipoxin A4 (LXA4). However, prostaglandin E2 (PGE2) was related to systemic disease severity during E. coli mastitis. Moreover, reduced somatic cell count (SCC), fewer circulating basophils, increased concentration of tumor necrosis factor-α (TNF-α) and higher milk sodium and lower milk potassium concentrations were related to systemic disease severity. The thermal camera was capable of detecting 2-3°C temperature changes on udder skin surface of cows inoculated with E. coli. Peak of udder skin temperature occurred after peak of rectal temperature and appearance of local signs of induced E. coli mastitis. Although infrared thermography was a successful method for detecting the changes in udder skin surface temperature following intramammary challenge with E. coli, it did not show to be a promising tool for early detection of mastitis.
Highlights
The severity of coliform mastitis is of much more concern than its incidence [1]
In contrast to previous research [9,10], no correlation existed between the severity of experimental E. coli mastitis and preinfection concentrations of BHBA, glucose and non-esterified fatty acids (NEFA)
We showed that milk prostaglandin E2 (PGE2) and thromboxane B2 (TXB2) concentrations were increased after intramammary challenge of E. coli
Summary
The severity of coliform mastitis is of much more concern than its incidence [1]. Cow and environment are three interdependent factors which influence the mastitis susceptibility [1]. From the various bacterial virulence factors studied during Escherichia coli mastitis [2], only a few have been found to play an important role in the outcome of the disease. It has been accepted that the type of E. coli strain is not the main factor in classification of severity. The severity of bovine E. coli mastitis is mainly ranking based on severity after intramammary infection of E. coli is poorly understood during early lactation. Physiological factors have been mainly studied in multiparous cows ranging from second lactation to sixth lactation [6,7,8,9,10]
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