Abstract
Summary Fibroblasts from two progeria patients were tested for their capacity to repair γ-irradiated DNA. An immunofluorescence host cell reactivation (HCR) assay was employed to compare normal, progeria, and Xeroderma Pigmentosum strains. HCR decreased markedly over a three day period in confluent cultures, indicating that growth conditions can substantially effect the DNA repair observed in HCR of cultured fibroblasts. Using a variety of growth conditions, one progeria strain showed decreased HCR, while a second progeria strain showed normal HCR. These results suggest both that heterogeneity for DNA repair capacity exists among progeria fibroblast strains and that decreased DNA repair capacity is unlikely to be the basic genetic defect in progeria.
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