Variation of biomass and kinetic parameters for nitrifying species in the TNCU3 process at different aerobic hydraulic retention times

Abstract

In this study, the variation of biomass, kinetic parameters, and stoichiometric parameters for ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in TNCU3 process were explored at different aerobic hydraulic retention time (AHRT). The results indicated that the growth rate constants of AOB were 0.92, 0.88, and 0.95 days−1, respectively, meanwhile, those of NOB were 2.58 1.41, and 1.40 days−1, respectively, when AHRT was 5, 6, and 7 h. The lysis rate constants for AOB and NOB were 0.13 and 0.17 days−1, respectively. When AHRT was 5, 6, and 7 h, the yield coefficients of AOB were 0.20, 0.23, and 0.28 g COD g−1 N, respectively, meanwhile those of NOB were 0.23, 0.19, and 0.22 g COD g−1 N, respectively. The average percentage of AOB was 0.44, 0.61, and 0.64%, respectively, while that of NOB was 0.46, 0.61, and 0.74%, respectively. The relation between the biomass percentage of AOB and AHRT was in a good agreement with first type hyperbolic curve. The relation between the biomass percentage of NOB and AHRT was in a good agreement with seven types of curve including simple exponential curve, power exponential curve, and first type hyperbolic curve etc. When the AHRT increased from 5 to 7 h, the removal efficiency of NH4 +–N increased from 80.2 to 94.8%, or by 14.6%. Meanwhile, the removal efficiency of total nitrogen increased from 63.6 to 70.9%, or by 7.3%.