Variation in tolerance induction and oncogenicity due to strain of avian leukosis virus

Abstract

Chickens were inoculated as embryos or orally at hatching with various doses of four strains of avian leukosis virus (ALV) subgroup A (RAV-1, RPL40, RPL41 and RPL42). Viraemia, antibody and tumours in chickens of various groups were compared; ALV shedding was determined, but only in chickens inoculated with virus at hatching. Results indicate that 95% to 100% of chickens embryonally inoculated with 105 infectious units of virus were viraemic at hatching, regardless of the strain of virus used. However, the incidence of viraemia in groups of chickens embryonally inoculated with 100 infectious units of virus varied, depending on the strain of virus, from 5% to 72%. All embryonally inoculated chickens, that had detectable virus at hatching and survived to 16 weeks of age were immunologically tolerant to the virus. ALV-induced tumours ranged from 4% to 47% depending on the strain of virus. Chickens inoculated orally at hatching did not develop immuno-logical tolerance to the virus; 13% to 76% of these chickens, depending on the strain of virus, had antibody by 18 weeks of age. ALV shedding in albumen of eggs or cloacal swabs at 42 weeks of age varied from 7% to 9%. Data from this study indicate that the strain of ALV may influence induction of immunological tolerance and tumours in embryonally inoculated chickens and induction of antibody in nontolerantly infected chickens. The data also suggest that chickens that are viraemic at hatching are probably incapable of breaking tolerance and developing antibody.